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Corning Life Sciences
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R&D Systems
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Imedex LLC
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Placenta Growth Factor-1 Human Recombinant produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of approximately 34 kDa. The PLGF-1 is purified
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Recombinant Human Placenta Growth Factor-1 (PlGF-1) is a 19 kDa protein produced ininsect cellsas a homodimer containing 131 amino acid residues andwith a C-terminal His-tag (6x His).Human Placenta Growth Factor (PlGF) is a polypeptide growth
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Journal:
Article Title: SPARC (Secreted Protein Acidic and Rich in Cysteine) Induces Apoptosis in Ovarian Cancer Cells
doi:
Figure Lengend Snippet: Cell surface receptor binding assays of HOSE cells and ovarian cancer cells. A secreted SPARC-AP fusion protein consists of human SPARC and a thermostable human placental alkaline phosphatase (AP) was used to examine the presence of putative SPARC receptors in ovarian cells. As background control, a secretory AP protein produced by the 293T/pAPtag-4 stable cell line was also used in this assay. Cells incubated with culture medium containing SPARC-AP or AP proteins were washed, lysed, and assayed for AP activities. Increase in ligand binding is indicated by the increase in AP activity, which is quantified by measuring absorbance at 405 nm. HOSE1-15 cells showed high levels of binding, whereas the three ovarian cancer cell lines studied have less binding to the SPARC-AP fusion protein.
Article Snippet: The amplified DNA fragment was digested with Hind III and Bgl II, which was subsequently inserted in-frame upstream of the coding sequence of a
Techniques: Cell Surface Receptor Assay, Binding Assay, Produced, Stable Transfection, Incubation, Ligand Binding Assay, Activity Assay